None.

Blood is being shown throughout the anime, which centers around two siblings who's "Takashiro disease" makes them automatically thirsting for blood, especially the main male character.

Some flesh cutting and biting. Nothing too graphic.

Suicide is talked about.

Chapter three has the male character being slapped in the face by his aunt.

Drugs are being talked about, and shown, mostly in relation to the "Takashiro illness" and suicide.

This anime is about two siblings, who's "Takashiro disease" makes them thirsting for blood like vampires, especially the main male character. Some content may be disturbing for younger children.