Arachnophobia (1990)

A large spider from the jungles of South America is accidentally transported in a crate with a dead body to America where it mates with a local spider. Soon after, the residents of a small California town disappear as the result of spider bites from the deadly spider offspring. It's up to a couple of doctors aided by an insect exterminator to annihilate these eight-legged freaks before they take over the entire town.