Some references to sex during a comic sequence in which the boys partake in an impromptu sex education lesson as part of their studies.

Vague reference to hobos having "fun ideas" about what to do with little boys, mentioned as a deterrent to running away.

29:00-32:00 kids ask their uncle about sex and then there's crude dialog about the male extremity and sex.

47:40 a boy asks a man if he wants to take a woman's clothes off. Then repeats "boobies + Privates = babies"

59:00 a teenage boy and girl kiss.

At one point, an adult describes to kids about "hobos on trains who have lots of interesting ideas about how to have fun with little boys", culminating in telling the kids they'll "wind up chopped up and stuffed into an oil barrel". The children are visibly alarmed.